Explore a selection of key publications that provide foundational insights and advanced perspectives on Adverse Outcome Pathways (AOPs). These publications cover strategies, methodologies, and applications that support the development and implementation of AOPs in toxicology and risk assessment

• OECD, 2018. "Users' Handbook supplement to the Guidance Document for developing and assessing Adverse Outcome Pathways," OECD Series on Adverse Outcome Pathways, No. 1, OECD Publishing, Paris.
  • NB: Latest edition of User’s Handbook is available on AOP-Wiki website as OECD AOP Developers’ Handbook 2.7 (Released May 30, 2024)
• Villeneuve DL et al., 2014. “Adverse outcome pathway (AOP) development I: strategies and principles.” Toxicological Sciences vol. 142, no. 2 (2014): 312–320.
• Villeneuve DL et al., 2014. “Adverse outcome pathway development II: best practices.” Toxicological Sciences vol. 142, no. 2 (2014): 321–330.
• Knapen D et al., 2018. “Adverse outcome pathway networks I: Development and applications.” Environmental Toxicology and Chemistry vol. 37, no. 6 (2018): 1723–1733.
• Villeneuve DL et al., 2018. “Adverse outcome pathway networks II: Network analytics.” Environmental Toxicology and Chemistry vol. 37, no. 6 (2018): 1734–1748.
• Leist M et al., 2017. “Adverse outcome pathways: opportunities, limitations and open questions.” Archives of Toxicology vol. 91, no. 11 (2017): 3477–3505.
• Vinken M., 2013. “The adverse outcome pathway concept: a pragmatic tool in toxicology.” Toxicology vol. 312 (2013): 158–165. https://doi.org/10.1016/j.tox.2013.08.011.
• Vinken M et al., 2017. “Adverse outcome pathways: a concise introduction for toxicologists.” Archives of Toxicology vol. 91, no. 11 (2017): 3697–3707.
• Vinken M., 2016. “Adverse Outcome Pathways as Tools to Assess Drug-Induced Toxicity.” Methods in Molecular Biology vol. 1425 (2016): 325–337.
• Vinken M et al., 2014. “Adverse outcome pathways: hype or hope?” Archives of Toxicology vol. 88, no. 1 (2014): 1–2.
• Fay KA et al., 2017. “Practical approaches to adverse outcome pathway development and weight-of-evidence evaluation as illustrated by ecotoxicological case studies.” Environmental Toxicology and Chemistry vol. 36, no. 6 (2017): 1429–1449.
• Verhoeven A et al., 2024. “A quantitative weight-of-evidence method for confidence assessment of adverse outcome pathway networks: A case study on chemical-induced liver steatosis.” Toxicology vol. 505 (2024): 153814.
• Collier ZA et al., 2016. “A weight of evidence assessment approach for adverse outcome pathways.” Regulatory Toxicology and Pharmacology vol. 75 (2016): 46–57.
• Rycroft T et al., 2018. “Weight of Evidence Frameworks in Evaluation of Adverse Outcome Pathways.” In: Garcia-Reyero, N., Murphy, C. (eds) A Systems Biology Approach to Advancing Adverse Outcome Pathways for Risk Assessment, Springer, Cham.
• Perkins EJ et al., 2019. “Chemical hazard prediction and hypothesis testing using quantitative adverse outcome pathways.” ALTEX vol. 36, no. 1 (2019): 91–102.
• Ankley et al., 2010 “Adverse outcome pathways: a conceptual framework to support ecotoxicology research and risk assessment.” Environmental toxicology and chemistry vol. 29,3 (2010): 730-41.
• Paini et al., 2022 “Towards a qAOP framework for predictive toxicology - Linking data to decisions.” Computational toxicology, vol. 21 (2022): 100195.
• Chemical Research in Toxicology and Environmental Science & Technology have compiled a Virtual Issue with articles from 2014 to 2016 related to AOPs.